How to ensure the accuracy of the 5ml/10ml Universal Pipette Tip when pipetting and transferring liquids?
Ensuring the accuracy of the
5ml/10ml Universal Pipette Tip in absorbing and transferring liquids is a crucial part of experimental operations. Here are some key steps and considerations to ensure accuracy:
Calibration check: Regularly perform calibration checks on the Pipette Tip to ensure that the amount of liquid it absorbs and releases meets the preset standards. This can be accomplished by comparison with a known volume of liquid sample.
Correct Operation: When using the Pipette Tip, the operating instructions should be strictly followed. Make sure the Pipette Tip is inserted vertically into the liquid to avoid inaccuracy in aspiration due to tilt or improper angle. At the same time, when releasing liquid, a stable movement should be maintained to avoid errors caused by being too fast or too slow.
Avoid bubbles: When sucking liquids, pay special attention to avoid the generation of bubbles. Air bubbles may cause less liquid to be aspirated, affecting accuracy. Therefore, you should ensure that the Pipette Tip is completely immersed in the liquid and draw slowly to avoid the generation of air bubbles.
Liquid viscosity and temperature: Liquids of different viscosities and temperatures may affect the pipette tip's pick-up and transfer performance. Therefore, before operation, you should understand the characteristics of the liquid and adjust the operation method or choose a suitable Pipette Tip as needed.
Keep it clean: The cleanliness of your pipette tip also has a significant impact on its accuracy. The Pipette Tip should be cleaned regularly to prevent contamination and residue from interfering with the suction and transfer of liquids.
Correct use range: Each Pipette Tip has its specific suction and transfer range. During operation, you should ensure that the amount of liquid sucked is within the applicable range of the Pipette Tip to ensure its accuracy.
Avoid cross-contamination: When handling multiple samples, care should be taken to avoid cross-contamination. Use a disposable Pipette Tip or clean and disinfect a reusable Pipette Tip thoroughly after each use to ensure accuracy with every sample.
In summary, through regular calibration, correct operation, avoiding air bubbles, paying attention to liquid characteristics, maintaining cleanliness, correct use range, and avoiding cross-contamination, the accuracy of the 5ml/10ml Universal Pipette Tip can be ensured when absorbing and transferring liquids.