96-well PCR Plates

Raised Semi-skirted 96-well PCR Plates

Raised Semi-skirted 96-well PCR Plates are designed to provide a high degree of accuracy and precision in PCR applications. These plates feature raised rims that prevent cross-contamination between wells and minimize the risk of sample loss. Our Raised Semi-skirted 96-well PCR Plates are designed for using it with the Applied Biosystems™ Fast PCR System. These plates are made from high-quality polypropylene and feature a raised semi-skirt design for maximum sealing efficiency.

Features and Benefits：

Raised rim design: The raised rims of these plates prevent cross-contamination between wells and minimize the risk of sample loss.
Well fit with Applied Biosystems™ Fast PCR System.
Clear well identification: The clear well identification on the plates makes it easy to identify the wells, reducing the risk of errors.
DNase/RNase-free and non-pyrogenic: These plates are free from DNase, RNase, and pyrogens, making them suitable for use in molecular biology applications.
Barcoding available: All plates can be provided with standard barcoding or barcoded to customer specifications for fully automated applications.

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Specifications

Well format:	96 wells
Well working volume:	0.1ml/0.2ml
Material:	USP Class VI medical grade Polypropylene (PP)
Skirt type:	Semi-skirted
Raised rim height:	1.2 mm
DNase/RNase-free and non-pyrogenic

Semi-skirted 96-well PCR plates are commonly used in molecular biology and genetic research for PCR (Polymerase Chain Reaction) applications. These plates are designed to hold PCR reactions in a convenient and organized format. Here's some information about raised semi-skirted 96-well PCR plates:
Well Configuration: These plates typically have 96 wells arranged in an 8x12 grid. Each well can hold a small volume of reaction mixture, usually in the range of 0.2 to 0.3 milliliters.
Raised Semi-Skirt: The term "semi-skirted" refers to the design of the plate's sides. Unlike fully skirted plates that have a skirt that goes all the way to the bottom, semi-skirted plates have a shorter skirt that only extends partially down the sides of the wells. This design allows for better heat transfer and compatibility with a wider range of thermal cyclers.
Raised Rim: The "raised" feature implies that the rim around the top of the plate is higher or thicker compared to non-raised PCR plates. This raised rim helps to minimize the risk of contamination and allows for better sealing when using adhesive or heat-sealing films.
Materials: PCR plates are typically made of high-quality polypropylene, which is compatible with the temperature cycling required for PCR. The plates are also designed to be resistant to chemical reactions, ensuring that they do not interfere with the PCR process.
Compatibility: Raised semi-skirted 96-well PCR plates are compatible with most thermal cyclers, ensuring a snug fit for a secure seal during PCR cycling.
Applications: These plates are used for various molecular biology applications, including PCR, qPCR (quantitative PCR), DNA amplification, sample storage, and other high-throughput nucleic acid analyses.
Sample Volume: The well volume of these plates may vary but is typically in the range of 100-200 microliters.
Identification: Most plates are barcoded for easy tracking and identification.
When using raised semi-skirted 96-well PCR plates, it's important to ensure that they are compatible with your specific thermal cycler and PCR reagents. Proper sealing, labeling, and organization of the plates are also essential for accurate and reproducible results in molecular biology experiments.

Applications
Our Raised Semi-skirted 96-well PCR Plates are ideal for a wide range of fast PCR applications, including:
- Gene expression analysis
- Pathogen detection
- DNA sequencing
- SNP genotyping
- And more!
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About

Zhejiang ZSup Medical Technology Co., Ltd.

Zhejiang ZSup Medical Technology Co., Ltd was established in 2018, Is a Raised Semi-skirted 96-well PCR Plates Manufacturers and Raised Semi-skirted 96-well PCR Plates Suppliers, by expert Teams of mold manufacturing, polymer materials, biomedicine, and IOT. We produce high-quality laboratory consumables in GMP qualified 100,000-level cleanliness level purification workshop. We also undertake manufacture of high-precision injection mold, customized bio supplies. We strive to provide one-stop solutions to our customers.
Manufacture of high-precision mold: Technical team own excellent experiences in producing of high-precision molds for bio supplies, particularly for thin-walled, multi-cavity, high-speed injection molds.
Manufacture of injection molding products: Top level production of laboratory supplies by world class QC and QA management, including
· General consumables: centrifuge tubes, tips, PCR, cell culture products, etc.;
· Customized consumables: all kinds of automated special biological experiment consumables;
· OEM customization: sharing equipment, molds, production lines, and available for OEM manufacture.
We offer a great balance of quality and affordability, while maintaining high-quality standards by leveraging economies of scale, optimizing production processes, and sourcing materials.

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Industry Knowledge Extension

How do Raised Semi-skirted 96-well PCR Plates optimize fluorescence detection sensitivity in PCR reactions?

In PCR reactions, Raised Semi-skirted 96-well PCR Plates significantly optimizes fluorescence detection sensitivity through its unique design features and premium material selection. This optimization not only improves the accuracy of experimental results, but also enhances the credibility of scientific research work.
First of all, the semi-skirted design is a highlight of the Raised Semi-skirted 96-well PCR Plates. This design reduces heat loss between the PCR plate and the thermal cycler, ensuring temperature accuracy and stability during the PCR reaction. A stable temperature environment is crucial for PCR amplification, as it helps maintain enzyme activity and promote DNA replication. Therefore, the half-skirt design can directly increase the intensity of the fluorescence signal, making the detection results more accurate and reliable.
Secondly, the uniformity of hole design is also a key factor in optimizing the sensitivity of fluorescence detection. The wells of Raised Semi-skirted 96-well PCR Plates are evenly spaced, which avoids the problems of optical path interference and signal crosstalk in fluorescence detection. This design ensures that the fluorescence signal of each well can be detected accurately and independently, thereby improving the accuracy and repeatability of detection results.
In addition, the high-quality light-transmitting materials used in PCR plates also play a key role in improving the sensitivity of fluorescence detection. These materials have good optical properties, ensuring that fluorescent signals are transmitted without excessive attenuation or interference. This makes the fluorescence signal clearer and stronger, further improving the sensitivity of fluorescence detection.
In addition to design features and material selection, the cleanliness and freedom from contamination of the PCR plate also have an important impact on fluorescence detection sensitivity. Thorough cleaning and disinfection of PCR plates before use can effectively remove residues and contaminants and ensure the accuracy and reliability of fluorescent signals. This not only helps improve the accuracy of experimental results, but also enhances the credibility of scientific research work.
In summary, Raised Semi-skirted 96-well PCR Plates significantly optimizes fluorescence detection sensitivity in PCR reactions through its unique design features, quality material selection, and good cleanliness. This optimization not only improves the accuracy of experimental results, but also provides scientific researchers with more reliable and credible experimental tools. In practical applications, scientific researchers can choose appropriate PCR plates according to experimental needs to obtain more accurate and reliable experimental results.

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