How do Filtered Pipette Tips reduce adsorption of RNA and other sensitive biomolecules?

Filtered pipette tips play a vital role in molecular biology and biotechnology, especially when working with RNA and other sensitive biomolecules. These biomolecules are easily damaged by physical, chemical or biological contamination, so it is crucial to reduce their loss and contamination during pipetting. Here are some ways to use filtered pipette tips to reduce adsorption of RNA and other sensitive biomolecules:
Choose the right filter membrane:
Choose filter membrane materials with low adsorption properties, such as specially treated plastic or silica gel.
Ensure that the filter membrane has the appropriate pore size to allow smooth passage of liquid while preventing contamination by particles and cell debris.
Optimize pipetting speed and pressure:
Slow and steady pipetting speed can reduce the adsorption of biomolecules on the filter membrane.
Avoid using excessive pressure or vacuum as this may increase the adsorption and destruction of biomolecules on the filter membrane.
Use RNase inhibitors:
For RNA samples, add an RNase inhibitor, such as DEPC (diethyl pyrocarbonate)-treated water or a specialized RNase inhibitor solution, to the sample before pipetting.
This helps reduce RNA degradation during pipetting.
Keep samples and pipette tips cool:
For sensitive biomolecules, maintaining low temperatures (such as 4°C) can reduce their degradation and adsorption.
Use refrigerated pipette tips and reagents, or use an ice box to maintain cold temperatures during pipetting.
Reduce pipetting times:
Minimize the number of pipetting times to reduce the contact and adsorption of biomolecules on the filter membrane.
Where possible, use larger pipetting volumes to reduce pipetting times.
Avoid solutions containing surfactants:
Certain surfactants may increase the adsorption of biomolecules to the filter membrane.
Try to avoid pipetting with solutions containing surfactants.
Replace pipette tips regularly:
Frequent replacement of pipette tips can reduce the adsorption of biomolecules due to aging or contamination of the filter membrane.
Make sure to check the integrity and cleanliness of pipette tips before use.
Use dedicated RNA processing tools:
For specific RNA applications, such as RNA sequencing or real-time quantitative PCR (qPCR), consider using pipettes and filters specifically designed for RNA.
These tools generally have lower RNA adsorption properties and higher sensitivity.
Training and experience:
Proper training and experience can improve the accuracy and consistency of pipetting techniques, thereby reducing the loss and contamination of biomolecules.
Regular skills assessment and feedback can help improve the overall performance of the lab.
Laboratory Cleaning and Hygiene:
Maintaining cleanliness and hygiene in the laboratory is essential to reduce contamination of biomolecules.
Clean laboratory surfaces, equipment, and tools regularly and follow good laboratory hygiene practices.